Anscombe, North the Welsh bolters
Wales head coach Warren Gatland has made six personnel changes from the team that knocked out hosts England. Besides Anscombe's inclusion to the starting XV, the team also sees star wing George North shift from the wing to outside centre to partner Jamie Roberts.
Anscombe forms part of a new-look back three alongside Alex Cuthbert and Liam Williams. Cuthbert will be making his 40th test appearance since making his debut against Australia back in 2011 whilst Williams returns from injury after missing the victory against Fiji.
Dan Biggar and Gareth Davies, who have collected the last two man-of-the-match awards in Wales' victories continue their partnership at flyhalf and scrumhalf respectively.
Paul James returns from injury to start in the front row alongside hooker Scott Baldwin and prop Samson Lee.Luke Charteris comes into the starting line-up to partner Alun Wyn Jones who makes his 99th Test appearance.
Captain Sam Warburton switches to blindside flank and features alongside fellow British and Irish Lions Justin Tipuric and Taulupe Faletau.
"Saturday is another huge encounter and both sides will be coming in with momentum and looking to top Pool A," said Gatland.
"We have been impressed with Australia so far in this competition and we know a step up is required at Twickenham this weekend. It is still all to play for in terms of the knockout phases.
"We've trained well this week, had a full squad to pick from and it is great to welcome back players from injury."
Ken Owens, Aaron Jarvis and Tomas Francis are named as the front-row replacements with Jake Ball and Ross Moriarty completing the forward contingent. Lloyd Williams, Rhys Priestland and James Hook provide the backline cover.
Wales: 15 Gareth Anscombe, 14 Alex Cuthbert, 13 George North, 12 Jamie Roberts, 11 Liam Williams, 10 Dan Biggar, 9 Gareth Davies, 8 Taulupe Faletau, 7 Justin Tipuric, 6 Sam Warburton (captain), 5 Alun Wyn Jones, 4 Luke Charteris, 3 Samson Lee, 2 Scott Baldwin, 1 Paul James.
Replacements: 16 Ken Owens, 17 Aaron Jarvis, 18 Tomas Francis, 19 Jake Ball, 20 Ross Moriarty, 21 Lloyd Williams, 22 Rhys Priestland, 23 James Hook.
Date: Saturday, October 10
Venue: Twickenham, London
Kick-off: 16.45 (15.45 GMT, 02.45 AEST Sunday, October 11)
Referee: Craig Joubert (South Africa)
Assistant referees: Jérôme Garcès (France), Stuart Berry (South Africa)
TMO: Shaun Veldsman (South Africa)
